PICNIC Lyrics

In the world of diagnostics there's a new way

CRISPR-Cas is breaking through leading the fray

Type V effectors with precision they seek

To find nucleic markers it's innovation we speak

PAM sites were the limit holding us down

But now we've found a way to turn it around

High heat high pH separating the strands

PICNIC's the method now we're taking a stand

Oh it's a PICNIC in the lab tonight

PAM-free detection shining bright

For HIV and HCV we're on the quest

With Cas12 enzymes we bring out the best

From double to single strands we break the chain

A ten-minute miracle no more PAM constraint

Cas12a Cas12b and Cas12i

In a PAM-free world we can reach the sky

High pH keeps the strands apart

Trans-cleavage activated it's a work of art

Detecting mutations drug-resistant strains

With PICNIC diagnostics are never the same

Oh it's a PICNIC in the lab tonight

PAM-free detection shining bright

For HIV and HCV we're on the quest

With Cas12 enzymes we bring out the best